User Tools

Site Tools


grain_spawn

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
grain_spawn [2020/10/22 23:28]
mwikiadmin
grain_spawn [2020/10/23 01:16] (current)
mwikiadmin
Line 17: Line 17:
  
  
-Grain Spawn Standard Operating Procedures: 
-Spawn Preparation 
-Penn State Spawn Lab Procedures for Making Spawn 
  
-In the spawn-production process, mycelium from a mushroom culture is placed onto steam-sterilized grain, and in time the mycelium completely grows through the grain. This grain/​mycelium mixture is called spawn, and spawn is used to "​seed"​ mushroom compost. 
-RYE GRAIN SPAWN 
-250 ml Flasks 
-50 ml beaker level full of rye grain 
-1/2 tsp. Calcium Carbonate, powder (lime) 
-1/4 tsp. Calcium Sulfate (gypsum) 
-60 ml warm water 
-Autoclave 35 minutes at 121°C - Fast Exhaust 
----------------------------------------------------------------- 
-1000 ml Flasks 
-250 ml beaker level full of rye grain 
-2 tsp. Calcium Carbonate, powder (lime) 
-1 tsp. Calcium Sulfate (gypsum) 
-220 ml warm water 
-Autoclave 45 minutes at 121°C - Fast Exhaust 
  
-SHIITAKE RYE SPAWN +
-Shiitake mushrooms are produced from spawn which is the vegetative growth or pure culture mushroom mycelium on a suitable sterilized substrate such as various agars, grains or wood chips. Formulas listed below were developed for use in producing spawn for synthetic logs. Synthetic logs are usually produced by autoclaving supplemented wood chips in heat-resistant plastic bags containing a breather strip then aseptically inoculating the cooled substrate with spawn. We have successfully produced shiitake mushrooms from synthetic logs inoculated with spawn manufactured as outlined below. +
-500 ml Flasks +
-100 ml beaker level full of rye grain +
-50 ml beaker of hardwood sawdust +
-1/2 tsp Calcium Sulfate (gypsum) +
-120 ml of warm tap water +
----------------------------------------------------------------------------- +
-1000 ml Flasks +
-250 ml beaker level full of rye grain +
-100 ml beaker level full of hardwood sawdust +
-1 tsp Calcium Sulfate (gypsum) +
-230 ml warm tap water +
-Use cotton plugs and paper bags over plugs and autoclave for 45 minutes at 121°C - Fast Exhaust+
  
  
grain_spawn.txt · Last modified: 2020/10/23 01:16 by mwikiadmin