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sop_rye_grain_spawn [2020/10/23 14:12]
mwikiadmin
sop_rye_grain_spawn [2020/10/23 14:14] (current)
mwikiadmin
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 Add all ingredients and seal your canning jar or grow bag, and mix the ingredients gently. Add all ingredients and seal your canning jar or grow bag, and mix the ingredients gently.
  
-If using a canning jar, make sure to twist the ring hand tight. It should be airtight and there is no concern of overpressurizing the jar or having it hold vacuum because of the fresh-air-exchange port that allows the jar to breath. Put a piece of foil over the top of the jar so that you can minimize the risk of contaminating the top of the lid from when you transfer it out of the pressure cooker into your ((Still_Air_Box)) ​or ((Laminar_flow_hood)) ​for inoculation. ​+If using a canning jar, make sure to twist the ring hand tight. It should be airtight and there is no concern of overpressurizing the jar or having it hold vacuum because of the fresh-air-exchange port that allows the jar to breath. Put a piece of foil over the top of the jar so that you can minimize the risk of contaminating the top of the lid from when you transfer it out of the pressure cooker into your [[still_air_box|still air box]] or [[laminar_flow_hood|Laminar flow hood]] ​for inoculation. ​
  
 If using a mushroom bag, try to push out as much air as possible, and seal the top with binder clips, a hair iron, rubber band, or even tie the top off in a nice tight knot.  If using a mushroom bag, try to push out as much air as possible, and seal the top with binder clips, a hair iron, rubber band, or even tie the top off in a nice tight knot. 
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 **Step 5** Inoculate the sterilized grain **Step 5** Inoculate the sterilized grain
-Allow the sterilized grain to cool to room temperature. Transfer the sterilized jar or bag to your ((Still_Air_Box)) ​or ((Laminar_flow_hood)) ​and using a sterile syringe tip, inject your liquid culture through the self-healing inoculation port.  +Allow the sterilized grain to cool to room temperature. Transfer the sterilized jar or bag to your [[still_air_box|still air box]] or [[laminar_flow_hood|Laminar flow hood]]and using a sterile syringe tip, inject your liquid culture through the self-healing inoculation port.  
-Usually, I use an ((alcohol_burner)) to get the steel syringe tip wet hot, cool it slightly with a single-use alcohol wipe, squirt out a few drops to clear the barrel of any microbials that might have survived the flame and alcohol, before injecting into the sterilized grain. ​+Usually, I use an alcohol burner((alcohol_burner)) to get the steel syringe tip wet hot, cool it slightly with a single-use alcohol wipe, squirt out a few drops to clear the barrel of any microbials that might have survived the flame and alcohol, before injecting into the sterilized grain. ​
  
 If your syringe tip is long enough, try to distribute the liquid culture in different spots of the grain so the entire mass will colonize more quickly. If your syringe tip is long enough, try to distribute the liquid culture in different spots of the grain so the entire mass will colonize more quickly.
  
 **Step 6** Wait for colonization **Step 6** Wait for colonization
-Depending on your ambient conditions and your particular cultivar , you should begin to see fuzzy white mycelium growth within 2-4 weeks. The more fully colonized the grain is, the more success you will have when you ((transplant)) the colonized grain to your grow substrate. ​+Depending on your ambient conditions and your particular cultivar , you should begin to see fuzzy white mycelium growth within 2-4 weeks. The more fully colonized the grain is, the more success you will have when you transplant((transplant)) the colonized grain to your grow substrate. ​
  
sop_rye_grain_spawn.txt · Last modified: 2020/10/23 14:14 by mwikiadmin